The prevalence of positive urine cultures in 100 dogs with an inactive urine sediment

  • Rachel Liebelt
  • Armi Pigott

Published:

2019-12-19

Share
Open Access Logo

DOI

https://doi.org/10.18849/ve.v4i4.273

Abstract

Objective: The purpose of this study was to determine the prevalence of positive quantitative urine cultures in dogs with an inactive urine sediment.

Background: A urinalysis is a useful screening tool for the evaluation of evidence of bacterial cystitis and a quantitative urine culture is used for definitive diagnosis.

Evidentiary value: A retrospective chart review from June 2012 to December 2017 at three private practice emergency and specialty referral hospitals examined urine samples obtained from 100 client-owned dogs.

Methods: The signalment and clinicopathologic data was recorded for all canine patients that had urine samples obtained by cystocentesis that had an inactive sediment exam on urinalysis and subsequent quantitative urine cultures were performed.

Results: The prevalence of positive quantitative urine cultures in all dogs with an inactive urine sediment at the aforementioned institutions was 6% (6/100). Escherichia coli was the most commonly isolated bacteria.

Conclusion: Given the low prevalence of positive quantitative urine cultures in dogs with an inactive urine sediment and current guideline recommendations for management of subclinical bacteriuria, we do not recommend urine cultures for dogs without lower urinary tract signs. Further prospective study of patient subgroups, as well as controlled studies evaluating urine sample handling techniques using methods available to private practice practitioners are sorely needed.

Application: Emergency and general practicing veterinarians should consider a quantitative urine culture for dogs with lower urinary tract signs, even with an inactive sediment examination, and on a case-by-case basis for dogs with pertinent systemic diseases or known risk factors for bacterial cystitis.

 

Open Access Peer Reviewed

References

Acierno, M.J., Partyka, M., Waite, K., Cunha, A. & Mitchell, M.A. ‘Effects of refrigeration of clinical canine urine samples on quantitative bacterial culture’. Journal of the American Veterinary Medical Association (2018); 253(2): 177–180. DOI: https://doi.org/10.2460/javma.253.2.177

Comer, K.M. & Ling, G.V. ‘Results of urinalysis and bacterial culture of canine urine obtained by antepubic cystocentesis, catheterization and the midstream voided methods’. Journal of the American Veterinary Medical Association (1981); 179: 891–895.

Ettinger, S.J., Feldman, E.C. & Côté, E. Textbook of Veterinary Internal Medicine. 8th ed. St. Louis: Elsevier, Inc. (2017).

Forrester, S.D., Troy, G.C., Dalton, M.N., Huffman, J.W. & Holtzman, G. Retrospective Evaluation of Urinary Tract Infection in 42 Dogs with Hyperadrenocorticism or Diabetes Mellitus or Both’. Journal of Veterinary Internal Medicine (1999); 13: 557–560. DOI: https://doi.org/10.1111/j.1939-1676.1999.tb02209.x

Foster, J.D., Krishnan, H. & Cole, S. ‘Characterization of subclinical bacteriuria, bacterial cystitis, and pyelonephritis in dogs with chronic kidney disease’. Journal of the American Veterinary Medical Association (2018); 252(10): 1257–1262. DOI: https://doi.org/10.2460/javma.252.10.1257

Freshman, J.L., Reif, J.S., Allen, T.A. & Jones, R.L. ‘Risk factors associated with urinary tract infection in female dogs’. Preventive Veterinary Medicine (1989); 7(2): 59–67. DOI: https://doi.org/10.1016/0167-5877(89)90037-8

Hall, J.L., Holmes, M.A. & Baines, S.J. ‘Prevalence and antimicrobial resistance of canine urinary tract pathogens’. Vet Record (2013); 173(22): 549. DOI: http://dx.doi.org/10.1136/vr.101482

Neely, J.G., Hartman, J.M., Forsen, J.W. Jr. & Wallace, M.S. Tutorials in clinical research: VII. Understanding comparative statistics (contrast)-part B: application of T-test, Mann-Whitney U, and chi-square. Laryngoscope. (2003); 113(10):1719–1725. DOI: https://doi.org/10.1097/00005537-200310000-00011

Padilla, J., Osborne, C.A. & Ward, G.E. ‘Effects of storage time and temperature on quantitative culture of canine urine’. Journal of the American Veterinary Medical Association (1981); 178: 1077–1081.

Patterson, C.A., Bishop, M.A., Pack, J.D., Cook, A.K. & Lawhon, S.D. ‘Effects of processing delay, temperature, and transport tube type on results of quantitative bacterial cultures of canine urine’. Journal of the American Veterinary Medical Association (2016); 248(2): 183–187. DOI: https://doi.org/10.2460/javma.248.2.183

Vetter, T.R. Fundamentals of Research Data and Variables: The Devil Is in the Details. Anesthesia & Analgesia. (2017); 125(4):1375–1380. DOI: http://dx.doi.org/10.1213/ANE.0000000000002370

Weese, S.J., Blondeau, J., Boothe, D., Guardabassi, L.D., Gumley, N., Papich, M., Jessen, L.R., Lappin, M., Rankin, S., Westropp, J.L. & Sykes, J. ‘International Society for Companion Animal Infectious Diseases (ISCAID) guidelines for the diagnosis and management of bacterial urinary tract infections in dogs and cats’. The Veterinary Journal (2019); 247: 8–25. DOI: https://doi.org/10.1016/j.tvjl.2019.02.008

1,100

Save

642

View

Themes by Openjournaltheme.com